Baculovirus-based expression of an insect viral protein in 12 different insect cell lines
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منابع مشابه
Cloning and Expression of Simian Rotavirus Spike Protein (VP4) in Insect Cells by Baculovirus Expression System
Background: VP4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. VP4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. Methods: Simian rotavirus SA11 gene 4 cDNA was cloned into a cloning plasmid pDONRTM by recombination reaction using clonase II enzyme mix. The resulting clone was c...
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The BEVS continues to evolve as a powerful, flexible tool for molecular biology, protein function, and biomedical research. Future developments offer the promise of replacement of hazardous chemical insecticides with environmentally safe biopesticides, construction of baculovirus vectors which encode genes for specific post-translational modifications, and establishment of efficient, stably tra...
متن کاملcloning and expression of simian rotavirus spike protein (vp4) in insect cells by baculovirus expression system
background: vp4 protein is as spikes on rotavirus outer capsid shell which is responsible for virus attachment to the host. vp4 induces production of neutralizing antibodies which could be used for serotyping of different isolates. methods: simian rotavirus sa11 gene 4 cdna was cloned into a cloning plasmid pdonrtm by recombination reaction using clonase ii enzyme mix. the resulting clone was c...
متن کاملA Model to Study the Phenotypic Changes of Insect Cell Transfection by Copepod Super Green Fluorescent Protein (cop-GFP) in Baculovirus Expression System
Background: Baculovirus expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor transfection efficiency or protein expression levels in insect cells. Methods: The aim of this study was to construct a baculovirus expression vector encoding a copepod super green fluore...
متن کاملCodon Usage in Signal Sequences Affects Protein Expression and Secretion Using Baculovirus/Insect Cell Expression System.
By introducing synonymous mutations into the coding sequences of GP64sp and FibHsp signal peptides, the influences of mRNA secondary structure and codon usage of signal sequences on protein expression and secretion were investigated using baculovirus/insect cell expression system. The results showed that mRNA structural stability of the signal sequences was not correlated with the protein produ...
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ژورنال
عنوان ژورنال: In Vitro Cellular and Developmental Biology--Animal
سال: 2005
ISSN: 1071-2690,1543-706X
DOI: 10.1290/0412081